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KMID : 1025520080500010045
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Journal of Animal Science and Technology
2008 Volume.50 No. 1 p.45 ~ p.56
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Postnatal Ontogeny of Expression of Monocarboxylate Transporters (MCTs) and Two Regulatory Proteins, Basigin and Embigin, in The Epididymis of Male Rat
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Lee Ki-Ho
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Abstract
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In the present study, real-time PCR was performed to evaluated expression of several isoforms of monocarboxylate transporters(MCTs) and two known MCT regulatory proteins, basigin (Bsg) and embigin, in the epididymis of the male reproductive tract during postnatal development. In addition, ERα�-mediated regulation of MCT1 expression in the epididymis was determined with estrogen receptor(ER) α� knockout(α�ERKO) mice by immunohistochemistry. Results from the current study demonstrated differential expression of MCT isoform(MCT 1, 2, 3, 4, and 8), Bsg, and embigin mRNAs in rat epididymis according to postnatal age and epididymal region. In addition, immunohistochemical study of MCT1 revealed the limited localization of MCT1 at apical area of corpus and caudal epididymis. The present study also showed that expression of MCT1 was not directly regulated by ERα�. The findings from the current study suggest that MCTs would involve in establishing adequate microenvironment for sperm maturation and storage in the epididymis, eventually leading to maintenance of male fertility.
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KEYWORD
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Epididymis, Monocarboxylate transporter, Basigin, Embigin
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